| Product name | Rat Pre-mRNA-processing factor 19 (PRPF19) ELISA Kit |
| Reactivity | Rat |
| Applications | ELISA |
| Applications notes | This Rat Pre-mRNA-processing factor 19 (PRPF19) ELISA Kit employs a two-site sandwich ELISA to quantitate PRPF19 in samples. An antibody specific for PRPF19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPF19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRPF19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPF19 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | PRPF19; NMP200; PRP19; PSO4; SNEV; UBOX4; hPSO4; PRP19/PSO4 homolog; PRP19/PSO4 pre-mRNA processing factor 19 homolog; nuclear matrix protein NMP200 related to splicing factor PRP19 |
| Kit components | • Rat Pre-mRNA-processing factor 19 microplate • Rat Pre-mRNA-processing factor 19 standard • Rat Pre-mRNA-processing factor 19 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Rat Pre-mRNA-processing factor 19 (PRPF19) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRPF19. No significant cross-reactivity or interference between Rat PRPF19 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair . PSO4 shares 38% amino acid identity with S. cerevisiae Pso4. SDS-PAGE indicated that PSO4 has an apparent molecular mass of 55 kD.PSO4 readily bound plasmid DNA substrates, including closed circular plasmid DNA, indicating that PSO4 binds in a sequence-independent manner and does not require free DNA ends for binding. PSO4 expression was induced 15- to 30-fold in a normal human fibroblast cell line by gamma irradiation and chemical mutagens, but not by ultraviolet irradiation. Loss of PSO4 expression induced by small interfering RNA resulted in accumulation of double-strand breaks, apoptosis, and decreased cell survival after DNA damage. |
| Gene ID | 246216 |
| Alternative | PRPF19; NMP200; PRP19; PSO4; SNEV; UBOX4; hPSO4; PRP19/PSO4 homolog; PRP19/PSO4 pre-mRNA processing factor 19 homolog; nuclear matrix protein NMP200 related to splicing factor PRP19 |
| Accession | Q9JMJ4 |
Fig.1. Rat Pre-mRNA-processing factor 19 (PRPF19) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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