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Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit

Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit

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Specification

Product name Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit employs a two-site sandwich ELISA to quantitate HCN3 in samples. An antibody specific for HCN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHCN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HCN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative HCN3; KIAA1535; MGC131493; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

Product Properties

Kit components • Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 microplate
• Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 standard
• Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat HCN3. No significant cross-reactivity or interference between Rat HCN3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background HCN3 conducted both potassium and sodium ions with a 3:1 preference for potassium ions. HCN3 bound cAMP, but unlike other HCNs, its activity was not modulated by intracellular cAMP.The cDNA contains a repetitive element in its 3-prime proximal region, and the deduced protein contains 703 amino acids. RT-PCR ELISA detected high HCN3 expression in adult liver and kidney and fetal liver and brain, intermediate expression in adult whole brain, lung, pancreas, spleen, testis, ovary, and all specific brain regions examined, and little to no expression in heart and skeletal muscle.Expression was high in fetal brain and adult cerebellum and intermediate in nucleus accumbens, thalamus, and pituitary gland, with little to no expression in other brain regions examined.
Gene ID 114245
Alternative HCN3; KIAA1535; MGC131493; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
Accession Q9JKA8

Image & description

Fig.1. Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) Standard Curve.

Fig.1. Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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