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Rat Polyserase-2 (PRSS36) ELISA Kit

Rat Polyserase-2 (PRSS36) ELISA Kit

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Specification

Product name Rat Polyserase-2 (PRSS36) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Polyserase-2 (PRSS36) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS36 in samples. An antibody specific for PRSS36 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS36 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS36 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS36 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRSS36; FLJ90661; polyserase-2; polyserine protease-2

Product Properties

Kit components • Rat Polyserase-2 microplate
• Rat Polyserase-2 standard
• Rat Polyserase-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Polyserase-2 (PRSS36) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRSS36. No significant cross-reactivity or interference between Rat PRSS36 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The deduced 855-amino acid protein has a predicted molecular mass of 93 kD and contains 9 potential N-glycosylation sites. PRSS36 contains a signal peptide, followed by a propeptide domain, an active serine protease domain with the catalytic his, asp, and ser residues, and 2 serine protease domains predicted to be catalytically inactive. The first serine protease domain shares amino acid identity of 40 to 42% with gamma-1 tryptase (TPSG1), pancreasin, matriptase (ST14;), matriptase-2 (TMPRSS6), and polyserase-1 (TMPRSS9). Northern blot analysis detected a 5-kb transcript in fetal kidney and adult skeletal muscle, liver, placenta, and heart. A 2.2-kb transcript was detected in placenta, prostate, and colon and lung adenocarcinoma cell lines, and may be produced by alternative splicing.
Alternative PRSS36; FLJ90661; polyserase-2; polyserine protease-2
Accession Q5K2P9

Image & description

Fig.1. Rat Polyserase-2 (PRSS36) Standard Curve.

Fig.1. Rat Polyserase-2 (PRSS36) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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