|Product name||Rat Polypeptide YY (PYY) ELISA Kit|
|Applications notes||This Rat Polypeptide YY (PYY) ELISA Kit employs a two-site sandwich ELISA to quantitate PYY in samples. An antibody specific for PYY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYY bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Polypeptide YY microplate
• Rat Polypeptide YY standard
• Rat Polypeptide YY detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Polypeptide YY (PYY) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PYY. No significant cross-reactivity or interference between Rat PYY and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Peptide YY is a short (36 amino acid) protein released by cells in the ileum and colon in response to feeding. It is also known as PYY, Peptide Tyrosine Tyrosine, or Pancreatic Peptide YY3-36. Peptide YY is related to the pancreatic peptide family by having 18 of its 36 amino acids located in the same positions as pancreatic peptide. There are two major forms of Peptide YY: PYY1-36 and PYY3-36 which have PP fold structural motif. However, the most common form of circulating PYY immunoreactivity is PYY3-36 which binds to Y2 receptor (Y2R) of Y family receptors. Peptide YY3-36 (PYY) is a linear polypeptide consisting of 36 amino acids with structural homology to NPY and pancreatic polypeptide.|
Fig.1. Rat Polypeptide YY (PYY) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.