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Rat Polymeric immunoglobulin receptor (PIGR) ELISA Kit

Rat Polymeric immunoglobulin receptor (PIGR) ELISA Kit

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Specification

Product name Rat Polymeric immunoglobulin receptor (PIGR) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Polymeric immunoglobulin receptor (PIGR) ELISA Kit employs a two-site sandwich ELISA to quantitate PIGR in samples. An antibody specific for PIGR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIGR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIGR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIGR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PIGR; FLJ22667; MGC125361; MGC125362; hepatocellular carcinoma associated protein TB6

Product Properties

Kit components • Rat Polymeric immunoglobulin receptor microplate
• Rat Polymeric immunoglobulin receptor standard
• Rat Polymeric immunoglobulin receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Polymeric immunoglobulin receptor (PIGR) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PIGR. No significant cross-reactivity or interference between Rat PIGR and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Polymeric immunoglobulin receptor is a Fc receptor which facilitates the secretion of IgA and IgM. The poly-Ig receptor is expressed on several glandular epithelia including those of liver and breast. It mediates transcellular transport of polymeric immunoglobulin molecules. It is a member of the immunoglobulin superfamily (Hood et al., 1985). The receptor has 5 units with homology to the variable (V) units of immunoglobulins and a transmembrane region, which also has some homology to certain immunoglobulin variable regions.
Gene ID 100328593
Alternative PIGR; FLJ22667; MGC125361; MGC125362; hepatocellular carcinoma associated protein TB6
Accession P01832

Image & description

Fig.1. Rat Polymeric immunoglobulin receptor (PIGR) Standard Curve.

Fig.1. Rat Polymeric immunoglobulin receptor (PIGR) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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