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Rat Platelet-derived growth factor-B (PDGF-B) ELISA Kit

Rat Platelet-derived growth factor-B (PDGF-B) ELISA Kit

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Specification

Product name Rat Platelet-derived growth factor-B (PDGF-B) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Platelet-derived growth factor-B (PDGF-B) ELISA Kit employs a two-site sandwich ELISA to quantitate PDGFB in samples. An antibody specific for PDGFB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGFB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PDGFB; FLJ12858; PDGF2; SIS; SSV; c-sis; PDGF; B chain; Platelet-derived growth factor; beta polypeptide (oncogene SIS); becaplermin; platelet-derived growth factor 2; platelet-derived growth factor beta; plate

Product Properties

Kit components • Rat Platelet-derived growth factor-B microplate
• Rat Platelet-derived growth factor-B standard
• Rat Platelet-derived growth factor-B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Platelet-derived growth factor-B (PDGF-B) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PDGFB. No significant cross-reactivity or interference between Rat PDGFB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Platelet-derived growth factor subunit B is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.
Gene ID 24628
Alternative PDGFB; FLJ12858; PDGF2; SIS; SSV; c-sis; PDGF; B chain; Platelet-derived growth factor; beta polypeptide (oncogene SIS); becaplermin; platelet-derived growth factor 2; platelet-derived growth factor beta; plate
Accession Q05028

Image & description

Fig.1. Rat Platelet-derived growth factor-B (PDGF-B) Standard Curve.

Fig.1. Rat Platelet-derived growth factor-B (PDGF-B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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