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Rat Platelet antibody IgG (PA-IgG) ELISA Kit

Rat Platelet antibody IgG (PA-IgG) ELISA Kit

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Product name Rat Platelet antibody IgG (PA-IgG) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Platelet antibody IgG (PA-IgG) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PA-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated PA-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled PA-IgG and unlabeled PA-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PA-IgG in the sample. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PA-IgG

Product Properties

Kit components • Rat Platelet antibody IgG microplate
• Rat Platelet antibody IgG standard
• Rat Platelet antibody IgG detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Platelet antibody IgG (PA-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PA-IgG. No significant cross-reactivity or interference between Rat PA-IgG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Neonatal alloimmune thrombocytopenia results from the placental transfer of maternal platelet IgG antibodies (alloantibodies) formed against incompatible fetal platelet antigens. For example, HPA-1a (PLA-A1; Zw) negative mothers carrying an HPA-1a positive fetus develop the anti-HPA-1a antibodies that are found in 80% of neonatal alloimmune thrombocytopenia cases. Additional platelet antigens involved include HPA-5 (Br; Zv) (10% of cases), HPA-2 (Ko), HPA-3 (Bak; Lek), HPA-4 (Pen; Yuk), and HPA-6 (Ca; Tu). Involvement of HLA class I antibodies has been suspected in a few cases, but is not yet proven.
Alternative PA-IgG

Image & description

Fig.1. Rat Platelet antibody IgG (PA-IgG) Standard Curve.

Fig.1. Rat Platelet antibody IgG (PA-IgG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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