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Rat Platelet-activating factor receptor (PTAFR) ELISA Kit

Rat Platelet-activating factor receptor (PTAFR) ELISA Kit

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Product name Rat Platelet-activating factor receptor (PTAFR) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Platelet-activating factor receptor (PTAFR) ELISA Kit employs a two-site sandwich ELISA to quantitate PTAFR in samples. An antibody specific for PTAFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTAFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTAFR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTAFR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTAFR; PAFR; OTTHUMP00000004041

Product Properties

Kit components • Rat Platelet-activating factor receptor microplate
• Rat Platelet-activating factor receptor standard
• Rat Platelet-activating factor receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Platelet-activating factor receptor (PTAFR) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PTAFR. No significant cross-reactivity or interference between Rat PTAFR and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The platelet-activating factor receptor is a G-protein coupled receptor which binds platelet-activating factor.The PAF receptor shows structural characteristics of the rhodopsin gene family and binds platelet-activating factor (PAF). PAF is a phospholipid (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) that has been implicated as a mediator in diverse pathologic processes, such as allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes. PTAFR has 2 N-glycosylation sites, but unlike other rhodopsin family members, there is no N-glycosylation site in the N-terminal segment. Transfection of PTAFR into COS-7 cells resulted in expression of the receptor with an extracellular N terminus.
Gene ID 58949
Alternative PTAFR; PAFR; OTTHUMP00000004041
Accession P46002

Image & description

Fig.1. Rat Platelet-activating factor receptor (PTAFR) Standard Curve.

Fig.1. Rat Platelet-activating factor receptor (PTAFR) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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