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Rat Plasminogen activator inhibitor 2 (SERPINB2) ELISA Kit

Rat Plasminogen activator inhibitor 2 (SERPINB2) ELISA Kit

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Specification

Product name Rat Plasminogen activator inhibitor 2 (SERPINB2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Plasminogen activator inhibitor 2 (SERPINB2) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINB2 in samples. An antibody specific for SERPINB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SERPINB2; HsT1201; PAI; PAI-2; PAI2; PLANH2; plasminogen activator inhibitor 2; plasminogen activator inhibitor; type (arginine-serpin); serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member

Product Properties

Kit components • Rat Plasminogen activator inhibitor 2 microplate
• Rat Plasminogen activator inhibitor 2 standard
• Rat Plasminogen activator inhibitor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Plasminogen activator inhibitor 2 (SERPINB2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SERPINB2. No significant cross-reactivity or interference between Rat SERPINB2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Plasminogen activator inhibitor-2 (placental PAI) is a coagulation factor that inactivates tPA and urokinase. It is present in most cells, especially monocytes/macrophages. PAI-2 exists in two forms, a 60-kDa extracellular glycosylated form and a 43-kDa intracellular form. It is present only at detectable quantities in blood during pregnancy, as it is produced by the placenta, and may explain partially the increased rate of thrombosis during pregnancy. The majority of expressed PAI-2 remains unsecreted due to the presence of an inefficient internal signal peptide.the intron-exon arrangement of PAI2 is identical to that of chicken ovalbumin and Y genes and distinct from that of other members of the serpin superfamily.
Gene ID 60325
Alternative SERPINB2; HsT1201; PAI; PAI-2; PAI2; PLANH2; plasminogen activator inhibitor 2; plasminogen activator inhibitor; type (arginine-serpin); serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member
Accession P29524

Image & description

Fig.1. Rat Plasminogen activator inhibitor 2 (SERPINB2) Standard Curve.

Fig.1. Rat Plasminogen activator inhibitor 2 (SERPINB2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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