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Rat Pituitary tumor-transforming gene 1 protein-interacting protein (PTTG1IP) ELISA Kit

Rat Pituitary tumor-transforming gene 1 protein-interacting protein (PTTG1IP) ELISA Kit

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Specification

Product name Rat Pituitary tumor-transforming gene 1 protein-interacting protein (PTTG1IP) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Pituitary tumor-transforming gene 1 protein-interacting protein (PTTG1IP) ELISA Kit employs a two-site sandwich ELISA to quantitate PTTG1IP in samples. An antibody specific for PTTG1IP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTTG1IP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTTG1IP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTTG1IP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTTG1IP; C21orf1; C21orf3; PBF; PTTG-binding factor; pituitary tumor-transforming gene 1 protein-interacting protein; pituitary tumor-transforming gene protein-binding factor

Product Properties

Kit components • Rat Pituitary tumor-transforming gene 1 protein-interacting protein microplate
• Rat Pituitary tumor-transforming gene 1 protein-interacting protein standard
• Rat Pituitary tumor-transforming gene 1 protein-interacting protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Pituitary tumor-transforming gene 1 protein-interacting protein (PTTG1IP) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PTTG1IP. No significant cross-reactivity or interference between Rat PTTG1IP and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PTTG1IP, which directly binds to pituitary tumor-transforming gene 1 protein (PTTG1), facilitates the nuclear translocation of PTTG1 and potentiates the transcriptional activation of basic fibroblast growth factor by PTTG1. The gene product localizes to both the cytoplasm and nucleus. Its NLS is required for its own nuclear localization, the nuclear localization of PTTG1, and its interaction with PTTG1. The predicted 180-amino acid C21ORF3 protein has features of a type Ia integral membrane protein and contains the tetrapeptide YXRF, a motif observed in proteins internalized via coated pit-mediated endocytosis. Northern blot analysis detected a 2.69-kb C21ORF3 mRNA in all tissues examined.
Gene ID 365548
Alternative PTTG1IP; C21orf1; C21orf3; PBF; PTTG-binding factor; pituitary tumor-transforming gene 1 protein-interacting protein; pituitary tumor-transforming gene protein-binding factor
Accession Q6P767

Image & description

Fig.1. Rat Pituitary tumor-transforming gene 1 protein-interacting protein (PTTG1IP) Standard Curve.

Fig.1. Rat Pituitary tumor-transforming gene 1 protein-interacting protein (PTTG1IP) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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