| Product name | Rat Phosphorylated microtubule-associated protein tau (pMAPT /pTAU) ELISA Kit |
| Reactivity | Rat |
| Applications | ELISA |
| Applications notes | This Rat Phosphorylated microtubule-associated protein tau (pMAPT /pTAU) ELISA Kit employs a two-site sandwich ELISA to quantitate pMAPT /pTAU in samples. An antibody specific for pMAPT /pTAU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anypMAPT /pTAU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for pMAPT /pTAU is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of pMAPT /pTAU bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | pMAPT /pTAU; phosphorylated -- DDPAC; FLJ31424; FTDP-17; MAPTL; MGC138549; MSTD; MTBT1; MTBT2; PPND; TAU; G protein beta1/gamma2 subunit-interacting factor 1; microtubule-associated protein tau; isoform 4 |
| Kit components | • Rat Phosphorylated microtubule-associated protein tau microplate • Rat Phosphorylated microtubule-associated protein tau standard • Rat Phosphorylated microtubule-associated protein tau detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Rat Phosphorylated microtubule-associated protein tau (pMAPT /pTAU) ELISA Kit has high sensitivity and excellent specificity for detection of Rat pMAPT /pTAU. No significant cross-reactivity or interference between Rat pMAPT /pTAU and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Tau protein is a highly soluble microtubule-associated protein (MAP). In humans, these proteins are mostly found in neurons compared to non-neuronal cells. One of tau's main functions is to modulate the stability of axonal microtubules. Tau is not present in dendrites and is active primarily in the distal portions of axons where it provides microtubule stabilization but also flexibility as needed. This contrasts with STOP proteins in the proximal portions of axons which essentially lock down the microtubules and MAP2 that stabilizes microtubules in dendrites. The tau gene locates on chromosome 17q21, containing 16 exons. The major tau protein in the human brain is encoded by 11 exons. Exon 2, 3 and 10 are alternative spliced, allowing six combinations (2-3-10-; 2+3-10-; 2+3+10-; 2-3-10+; 2+3-10+; 2+3+10+). |
| Alternative | pMAPT /pTAU; phosphorylated -- DDPAC; FLJ31424; FTDP-17; MAPTL; MGC138549; MSTD; MTBT1; MTBT2; PPND; TAU; G protein beta1/gamma2 subunit-interacting factor 1; microtubule-associated protein tau; isoform 4 |
Fig.1. Rat Phosphorylated microtubule-associated protein tau (pMAPT /pTAU) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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