|Product name||Rat Phospholipid scramblase 1 (PLSCR1) ELISA Kit|
|Applications notes||This Rat Phospholipid scramblase 1 (PLSCR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLSCR1 in samples. An antibody specific for PLSCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLSCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLSCR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLSCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Phospholipid scramblase 1 microplate
• Rat Phospholipid scramblase 1 standard
• Rat Phospholipid scramblase 1 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Phospholipid scramblase 1 (PLSCR1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PLSCR1. No significant cross-reactivity or interference between Rat PLSCR1 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Scramblases are members of the general family of transmembrane lipid transporters known as flippases. The inner-leaflet facing the inside of the cell contains negatively-charged amino-phospholipids and phosphatidylethanolamine. The outer-leaflet, facing the outside environment, contains phosphatidylcholine and sphingomyelin. Scramblase is an enzyme, present in the cell membrane, that can transport (scramble) the negatively-charged phospholipids from the inner-leaflet to the outer-leaflet, and vice versa.Wiedmer et al. (2000) determined that the PLSCR1 gene contains 9 exons and spans about 30 kb. The first exon is untranslated. By deletion analysis of PLSCR1 promoter-reporter constructs, they found that 97% of the promoter activity resides between -95 bp in the 5-prime flanking region to +60 bp of the first exon.|
Fig.1. Rat Phospholipid scramblase 1 (PLSCR1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.