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Rat Perilipin-1 (Plin1/Peri/Plin) ELISA Kit

Rat Perilipin-1 (Plin1/Peri/Plin) ELISA Kit

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Specification

Product name Rat Perilipin-1 (Plin1/Peri/Plin) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Perilipin-1 (Plin1/Peri/Plin) ELISA Kit employs a two-site sandwich ELISA to quantitate Plin1/Peri/Plin in samples. An antibody specific for Plin1/Peri/Plin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPlin1/Peri/Plin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Plin1/Peri/Plin is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Plin1/Peri/Plin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative Plin1/Peri/Plin; PERI; PLIN; lipid droplet-associated protein

Product Properties

Kit components • Rat Perilipin-1 microplate
• Rat Perilipin-1 standard
• Rat Perilipin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Perilipin-1 (Plin1/Peri/Plin) ELISA Kit has high sensitivity and excellent specificity for detection of Rat Plin1/Peri/Plin. No significant cross-reactivity or interference between Rat Plin1/Peri/Plin and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Perilipin is a protein that coats lipid droplets in adipocytes, the fat-storing cells in adipose tissue. Perilipin acts as a protective coating from the body’s natural lipases, such as hormone-sensitive lipase,which break triglycerides into glycerol and free fatty acids for use in metabolism, a process called lipolysis. Perilipin is hyperphosphorylated by PKA following β-adrenergic receptor activation. Phosphorylated perilipin changes conformation, exposing the stored lipids to hormone-sensitive lipase-mediated lipolysis. Although PKA also phosphorylates hormone-sensitive lipase, which can increase PKA activity, the more than 50-fold increase in fat mobilization (triggered by epinephrine) is primarily due to perilipin phosphorylation.
Gene ID 280981
Alternative Plin1/Peri/Plin; PERI; PLIN; lipid droplet-associated protein
Accession Q9TUM6

Image & description

Fig.1. Rat Perilipin-1 (Plin1/Peri/Plin) Standard Curve.

Fig.1. Rat Perilipin-1 (Plin1/Peri/Plin) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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