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Rat Paxillin (PXN) ELISA Kit

Rat Paxillin (PXN) ELISA Kit

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Product name Rat Paxillin (PXN) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Paxillin (PXN) ELISA Kit employs a two-site sandwich ELISA to quantitate PXN in samples. An antibody specific for PXN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PXN; hCG_1778014; FLJ16691;

Product Properties

Kit components • Rat Paxillin microplate
• Rat Paxillin standard
• Rat Paxillin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Paxillin (PXN) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PXN. No significant cross-reactivity or interference between Rat PXN and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Paxillin is a signal transduction adaptor protein and should not be confused with the neurotoxin paxilline.Glenney and Zokas (1989) used an antiphosphotyrosine antibody to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and found a 76-kD protein that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells. Turner et al. (1990) purified this protein from chicken gizzard smooth muscle, and named it paxillin ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions.Paxillin migrates as a diffuse 65- to 70-kD band on SDS-PAGE. Salgia et al. (1995) stated that transmembrane integrin molecules connect the actin cytoskeleton to the extracellular matrix within focal adhesions.
Gene ID 360820
Alternative PXN; hCG_1778014; FLJ16691;
Accession Q66H76

Image & description

Fig.1. Rat Paxillin (PXN) Standard Curve.

Fig.1. Rat Paxillin (PXN) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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