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Rat Parathyroid hormone (PTH) ELISA Kit

Rat Parathyroid hormone (PTH) ELISA Kit

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Specification

Product name Rat Parathyroid hormone (PTH) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Parathyroid hormone (PTH) ELISA Kit employs a two-site sandwich ELISA to quantitate PTH in samples. An antibody specific for PTH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTH; PTH1; parathormone; parathyrin; parathyroid hormone 1

Product Properties

Kit components • Rat Parathyroid hormone microplate
• Rat Parathyroid hormone standard
• Rat Parathyroid hormone detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Parathyroid hormone (PTH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PTH. No significant cross-reactivity or interference between Rat PTH and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Parathyroid hormone is secreted by the parathyroid glands as a polypeptide containing 84 amino acids. It acts to increase the concentration of calcium (Ca2+) in the blood, whereas calcitonin (a hormone produced by the parafollicular cells (C cells) of the thyroid gland) acts to decrease calcium concentration. PTH acts to increase the concentration of calcium in the blood by acting upon parathyroid hormone receptor in three parts of the body: PTH half-life is approximately 4 minutes. It has a molecular mass of 9.4 kDa. PTH was one of the first hormones to be shown to use the G-protein, adenylyl cyclase second messenger system.Normal total plasma calcium level ranges from 8.5 to 10.2 mg/dL (2.12 mmol/L to 2.55 mmol/L).
Gene ID 24694
Alternative PTH; PTH1; parathormone; parathyrin; parathyroid hormone 1
Accession P04089

Image & description

Fig.1. Rat Parathyroid hormone (PTH) Standard Curve.

Fig.1. Rat Parathyroid hormone (PTH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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