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Rat P2Y purinoceptor 14 (P2RY14) ELISA Kit

Rat P2Y purinoceptor 14 (P2RY14) ELISA Kit

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Specification

Product name Rat P2Y purinoceptor 14 (P2RY14) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat P2Y purinoceptor 14 (P2RY14) ELISA Kit employs a two-site sandwich ELISA to quantitate P2RY14 in samples. An antibody specific for P2RY14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP2RY14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P2RY14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P2RY14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative P2RY14; GPR105; KIAA0001; P2Y14; G protein coupled receptor for UDP-glucose; G protein-coupled receptor 105; P2Y purinoceptor 14; P2Y(14) receptor; P2Y14 receptor

Product Properties

Kit components • Rat P2Y purinoceptor 14 microplate
• Rat P2Y purinoceptor 14 standard
• Rat P2Y purinoceptor 14 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat P2Y purinoceptor 14 (P2RY14) ELISA Kit has high sensitivity and excellent specificity for detection of Rat P2RY14. No significant cross-reactivity or interference between Rat P2RY14 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background P2Y purinoceptor 14 is a protein belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene.The predicted 338-amino acid protein contains transmembrane domains and a tyrosine kinase phosphorylation site.
Gene ID 171108
Alternative P2RY14; GPR105; KIAA0001; P2Y14; G protein coupled receptor for UDP-glucose; G protein-coupled receptor 105; P2Y purinoceptor 14; P2Y(14) receptor; P2Y14 receptor
Accession O35881

Image & description

Fig.1. Rat P2Y purinoceptor 14 (P2RY14) Standard Curve.

Fig.1. Rat P2Y purinoceptor 14 (P2RY14) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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