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Rat Nuclear pore complex protein Nup153 (NUP153) ELISA Kit

Rat Nuclear pore complex protein Nup153 (NUP153) ELISA Kit

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Specification

Product name Rat Nuclear pore complex protein Nup153 (NUP153) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Nuclear pore complex protein Nup153 (NUP153) ELISA Kit employs a two-site sandwich ELISA to quantitate NUP153 in samples. An antibody specific for NUP153 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNUP153 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NUP153 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NUP153 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NUP153; HNUP153; N153; nuclear pore complex protein hnup153

Product Properties

Kit components • Rat Nuclear pore complex protein Nup153 microplate
• Rat Nuclear pore complex protein Nup153 standard
• Rat Nuclear pore complex protein Nup153 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Nuclear pore complex protein Nup153 (NUP153) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NUP153. No significant cross-reactivity or interference between Rat NUP153 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. The protein encoded by NUP153 has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats.
Alternative NUP153; HNUP153; N153; nuclear pore complex protein hnup153
Accession P49791

Image & description

Fig.1. Rat Nuclear pore complex protein Nup153 (NUP153) Standard Curve.

Fig.1. Rat Nuclear pore complex protein Nup153 (NUP153) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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