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Rat Nuclear envelope pore membrane protein POM 121 (POM121) ELISA Kit

Rat Nuclear envelope pore membrane protein POM 121 (POM121) ELISA Kit

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Specification

Product name Rat Nuclear envelope pore membrane protein POM 121 (POM121) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Nuclear envelope pore membrane protein POM 121 (POM121) ELISA Kit employs a two-site sandwich ELISA to quantitate POM121 in samples. An antibody specific for POM121 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOM121 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POM121 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POM121 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative POM121; DKFZp586G1822; DKFZp586P2220; FLJ41820; KIAA0618; MGC3792; POM121A; OTTHUMP00000160224; P145; nuclear envelope pore membrane protein POM 121; nuclear pore membrane protein 121; nuclear pore membrane pro

Product Properties

Kit components • Rat Nuclear envelope pore membrane protein POM 121 microplate
• Rat Nuclear envelope pore membrane protein POM 121 standard
• Rat Nuclear envelope pore membrane protein POM 121 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Nuclear envelope pore membrane protein POM 121 (POM121) ELISA Kit has high sensitivity and excellent specificity for detection of Rat POM121. No significant cross-reactivity or interference between Rat POM121 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. POM121 encodes a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined.
Gene ID 113975
Alternative POM121; DKFZp586G1822; DKFZp586P2220; FLJ41820; KIAA0618; MGC3792; POM121A; OTTHUMP00000160224; P145; nuclear envelope pore membrane protein POM 121; nuclear pore membrane protein 121; nuclear pore membrane pro
Accession P52591

Image & description

Fig.1. Rat Nuclear envelope pore membrane protein POM 121 (POM121) Standard Curve.

Fig.1. Rat Nuclear envelope pore membrane protein POM 121 (POM121) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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