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Rat NADH-cytochrome b5 reductase 3 (CYB5R3) ELISA Kit

Rat NADH-cytochrome b5 reductase 3 (CYB5R3) ELISA Kit

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Specification

Product name Rat NADH-cytochrome b5 reductase 3 (CYB5R3) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat NADH-cytochrome b5 reductase 3 (CYB5R3) ELISA Kit employs a two-site sandwich ELISA to quantitate CYB5R3 in samples. An antibody specific for CYB5R3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYB5R3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYB5R3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYB5R3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CYB5R3; B5R; DIA1; NADH-cytochrome b5 reductase; OTTHUMP00000198574; OTTHUMP00000198665; diaphorase (NADH) (cytochrome b-5 reductase)

Product Properties

Kit components • Rat NADH-cytochrome b5 reductase 3 microplate
• Rat NADH-cytochrome b5 reductase 3 standard
• Rat NADH-cytochrome b5 reductase 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat NADH-cytochrome b5 reductase 3 (CYB5R3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CYB5R3. No significant cross-reactivity or interference between Rat CYB5R3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CYB5R3 encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in CYB5R3 cause methemoglobinemias.
Gene ID 25035
Alternative CYB5R3; B5R; DIA1; NADH-cytochrome b5 reductase; OTTHUMP00000198574; OTTHUMP00000198665; diaphorase (NADH) (cytochrome b-5 reductase)
Accession P20070

Image & description

Fig.1. Rat NADH-cytochrome b5 reductase 3 (CYB5R3) Standard Curve.

Fig.1. Rat NADH-cytochrome b5 reductase 3 (CYB5R3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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