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Rat Myosin-IXb (MYO9B) ELISA Kit

Rat Myosin-IXb (MYO9B) ELISA Kit

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Specification

Product name Rat Myosin-IXb (MYO9B) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Myosin-IXb (MYO9B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO9B in samples. An antibody specific for MYO9B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO9B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO9B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO9B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MYO9B; CELIAC4; MYR5; unconventional myosin IXb

Product Properties

Kit components • Rat Myosin-IXb microplate
• Rat Myosin-IXb standard
• Rat Myosin-IXb detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Myosin-IXb (MYO9B) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MYO9B. No significant cross-reactivity or interference between Rat MYO9B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MYO9B encodes a member of the myosin family of actin-based molecular motor heavy chain proteins. Myosin-IXb represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-9 (MYH9). Myosin-IXb has four IQ motifs located in the neck domain that bind calmodulin, which serves as a light chain. Myosin-IXb complex has a single-headed structure and exhibits processive movement on actin filaments toward the minus-end. Myosin-IXb also has rho-GTPase activity. Polymorphisms in MYO9B are associated with celiac disease and ulcerative colitis susceptibility. Multiple transcript variants encoding different isoforms have been found for MYO9B. 
Alternative MYO9B; CELIAC4; MYR5; unconventional myosin IXb
Accession Q63358

Image & description

Fig.1. Rat Myosin-IXb (MYO9B) Standard Curve.

Fig.1. Rat Myosin-IXb (MYO9B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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