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Rat Myeloperoxidase-antineutrophil cytoplasmic antibody IgG (MPO-ANCA-IgG) ELISA Kit

Rat Myeloperoxidase-antineutrophil cytoplasmic antibody IgG (MPO-ANCA-IgG) ELISA Kit

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Specification

Product name Rat Myeloperoxidase-antineutrophil cytoplasmic antibody IgG (MPO-ANCA-IgG) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Myeloperoxidase-antineutrophil cytoplasmic antibody IgG (MPO-ANCA-IgG) ELISA Kit employs a two-site sandwich ELISA to quantitate MPO-ANCA-IgG in samples. An antibody specific for MPO-ANCA-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPO-ANCA-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPO-ANCA-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPO-ANCA-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MPO-ANCA-IgG

Product Properties

Kit components • Rat Myeloperoxidase-antineutrophil cytoplasmic antibody IgG microplate
• Rat Myeloperoxidase-antineutrophil cytoplasmic antibody IgG standard
• Rat Myeloperoxidase-antineutrophil cytoplasmic antibody IgG detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Myeloperoxidase-antineutrophil cytoplasmic antibody IgG (MPO-ANCA-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MPO-ANCA-IgG. No significant cross-reactivity or interference between Rat MPO-ANCA-IgG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Anti-neutrophil cytoplasmic antibodies (ANCAs) are a group of autoantibodies, mainly of the IgG type, against antigens in the cytoplasm of neutrophil granulocytes (the most common type of white blood cell) and monocytes. They are detected as a blood test in a number of autoimmune disorders, but are particularly associated with systemic vasculitis, so called ANCA-associated vasculitides.
Alternative MPO-ANCA-IgG

Image & description

Fig.1. Rat Myeloperoxidase-antineutrophil cytoplasmic antibody IgG (MPO-ANCA-IgG) Standard Curve.

Fig.1. Rat Myeloperoxidase-antineutrophil cytoplasmic antibody IgG (MPO-ANCA-IgG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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