|Product name||Rat Myelin basic protein antibody (MBP-Ab) ELISA Kit|
|Applications notes||This Rat Myelin basic protein antibody (MBP-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MBP-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated MBP-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled MBP-Ab and unlabeled MBP-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of MBP-Ab in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Myelin basic protein antibody microplate
• Rat Myelin basic protein antibody standard
• Rat Myelin basic protein antibody detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Myelin basic protein antibody (MBP-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MBP-Ab. No significant cross-reactivity or interference between Rat MBP-Ab and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Myelin basic protein (MBP) is a protein believed to be important in the process of myelination of nerves in the central nervous system (CNS). Myelin Basic Protein (MBP) is the major constituent of the CNS myelin synthesized by oligodendrocytes and Schwann cells. MBP, PLP (proteolipid proteins) and MOG (myelin oligodendrocyte glycoprotein) are potential primary encephalitogenic target antigens in multiple sclerosis.MBP was initially sequenced in 1979 after isolation from myelin membranes. Since that time, knockout mice deficient in MBP have been developed which showed decreased amounts of CNS myelination and a progressive disorder characterized by tremors, seizures, and early death. The gene for MBP is on chromosome 18; the protein localizes to the CNS and to various cells of the hematopoietic system.|
Fig.1. Rat Myelin basic protein antibody (MBP-Ab) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.