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Rat Myc proto-oncogene protein (MYC) ELISA Kit

Rat Myc proto-oncogene protein (MYC) ELISA Kit

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Specification

Product name Rat Myc proto-oncogene protein (MYC) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Myc proto-oncogene protein (MYC) ELISA Kit employs a two-site sandwich ELISA to quantitate MYC in samples. An antibody specific for MYC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MYC; MRTL; bHLHe39; c-Myc; avian myelocytomatosis viral oncogene homolog; myc proto-oncogene protein; myc-related translation/localization regulatory factor; v-myc avian myelocytomatosis viral oncogene homo

Product Properties

Kit components • Rat Myc proto-oncogene protein microplate
• Rat Myc proto-oncogene protein standard
• Rat Myc proto-oncogene protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Myc proto-oncogene protein (MYC) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MYC. No significant cross-reactivity or interference between Rat MYC and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Myc (cMyc) is a protooncogene, which is overexpressed in a wide range of human cancers. When it is specifically-mutated, or overexpressed, it increases cell proliferation and functions as an oncogene. Myc gene encodes for a transcription factor that regulates expression of 15% of all genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). Myc belongs to Myc family of transcription factors, which also includes N-Myc and L-Myc genes. Myc-family transcription factors contain the bHLH/LZ (basic Helix-Loop-Helix Leucine Zipper) domain.Myc protein is a transcription factor that activates expression of a great number of genes through binding on consensus sequences (Enhancer Box sequences (E-boxes)) and recruiting histone acetyltransferases (HATs).
Gene ID 24577
Alternative MYC; MRTL; bHLHe39; c-Myc; avian myelocytomatosis viral oncogene homolog; myc proto-oncogene protein; myc-related translation/localization regulatory factor; v-myc avian myelocytomatosis viral oncogene homo
Accession P09416

Image & description

Fig.1. Rat Myc proto-oncogene protein (MYC) Standard Curve.

Fig.1. Rat Myc proto-oncogene protein (MYC) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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