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Rat Multiple PDZ domain protein (MPDZ) ELISA Kit

Rat Multiple PDZ domain protein (MPDZ) ELISA Kit

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Specification

Product name Rat Multiple PDZ domain protein (MPDZ) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Multiple PDZ domain protein (MPDZ) ELISA Kit employs a two-site sandwich ELISA to quantitate MPDZ in samples. An antibody specific for MPDZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPDZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPDZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPDZ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MPDZ; RP11-382H24.1; DKFZp781P216; FLJ25909; FLJ34626; FLJ90240; MUPP1;

Product Properties

Kit components • Rat Multiple PDZ domain protein microplate
• Rat Multiple PDZ domain protein standard
• Rat Multiple PDZ domain protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Multiple PDZ domain protein (MPDZ) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MPDZ. No significant cross-reactivity or interference between Rat MPDZ and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by MPDZ has multiple PDZ domains, which are hallmarks of protein-protein interactions. The encoded protein is known to interact with the HTR2C receptor and may cause it to clump at the cell surface. Multiple transcript variants encoding different isoforms have been found for MPDZ.
Gene ID 29365
Alternative MPDZ; RP11-382H24.1; DKFZp781P216; FLJ25909; FLJ34626; FLJ90240; MUPP1;
Accession O55164

Image & description

Fig.1. Rat Multiple PDZ domain protein (MPDZ) Standard Curve.

Fig.1. Rat Multiple PDZ domain protein (MPDZ) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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