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Rat Multidrug resistance protein 1 (ABCB1) ELISA Kit

Rat Multidrug resistance protein 1 (ABCB1) ELISA Kit

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Specification

Product name Rat Multidrug resistance protein 1 (ABCB1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Multidrug resistance protein 1 (ABCB1) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCB1 in samples. An antibody specific for ABCB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ABCB1; ABC20; CD243; CLCS; GP170; MDR1; MGC163296; P-GP; PGY1; ATP-binding cassette; subfamily B; member 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance; multidrug resistance protein 1

Product Properties

Kit components • Rat Multidrug resistance protein 1 microplate
• Rat Multidrug resistance protein 1 standard
• Rat Multidrug resistance protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Multidrug resistance protein 1 (ABCB1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ABCB1. No significant cross-reactivity or interference between Rat ABCB1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp) also known as multidrug resistance protein 1 (MDR1) or ATP-binding cassette sub-family B member 1 (ABCB1) or cluster of differentiation 243 (CD243) is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent efflux pump with broad substrate specificity. It exists in animals, fungi and bacteria and likely evolved as a defense mechanism against harmful substances. P-gp is extensively distributed and expressed in the intestinal epithelium where it pumps xenobiotics (such as toxins or drugs) back into the intestinal lumen, in liver cells where it pumps them into bile ducts, in the cells of the proximal tubule of the kidney where it pumps them into urine-conducting ducts, and in the capillary endothelial cells composing the blood–brain barrier and blood-testis barrier, where it pumps them back into the capillaries. P-gp is a glycoprotein that in humans is encoded by the ABCB1 gene. P-gp is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily. The normal excretion of xenobiotics back into the gut lumen by P-gp pharmacokinetically reduces the efficacy of some pharmaceutical drugs (which are said to be P-gp substrates). In addition, some cancer cells also express large amounts of P-gp, further amplifying that effect and rendering these cancers multidrug resistant. Many drugs inhibit P-gp, typically incidentally rather than as their main mechanism of action; some foods do as well. Any such substance can sometimes be called a P-gp inhibitor.
Alternative ABCB1; ABC20; CD243; CLCS; GP170; MDR1; MGC163296; P-GP; PGY1; ATP-binding cassette; subfamily B; member 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance; multidrug resistance protein 1
Accession P43245

Image & description

Fig.1. Rat Multidrug resistance protein 1 (ABCB1) Standard Curve.

Fig.1. Rat Multidrug resistance protein 1 (ABCB1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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