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Rat Monocyte chemotactic protein 3 (MCP-3) ELISA Kit

Rat Monocyte chemotactic protein 3 (MCP-3) ELISA Kit

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Specification

Product name Rat Monocyte chemotactic protein 3 (MCP-3) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Monocyte chemotactic protein 3 (MCP-3) ELISA Kit employs a two-site sandwich ELISA to quantitate CCL7 in samples. An antibody specific for CCL7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCL7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CCL7; FIC; MARC; MCP-3; MCP3; MGC138463; MGC138465; NC28; SCYA6; SCYA7; monocyte chemoattractant protein 3; monocyte chemotactic protein 3; small inducible cytokine A7 (monocyte chemotactic protein 3)

Product Properties

Kit components • Rat Monocyte chemotactic protein 3 microplate
• Rat Monocyte chemotactic protein 3 standard
• Rat Monocyte chemotactic protein 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Monocyte chemotactic protein 3 (MCP-3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CCL7. No significant cross-reactivity or interference between Rat CCL7 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). Due to CCL7 possessing two adjacent N-terminal cysteine residues in its mature protein, it is classified among the subfamily of chemokines known as CC chemokines. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. CCL7 is one of the most pluripotent chemokines, produced by certain tumor cell lines and by macrophages and acting on multiple cell types including monocytes, lymphocytes, eosinophils, basophils, dendritic cells, and natural killer cells.
Gene ID 287561
Alternative CCL7; FIC; MARC; MCP-3; MCP3; MGC138463; MGC138465; NC28; SCYA6; SCYA7; monocyte chemoattractant protein 3; monocyte chemotactic protein 3; small inducible cytokine A7 (monocyte chemotactic protein 3)
Accession Q9QXY8

Image & description

Fig.1. Rat Monocyte chemotactic protein 3 (MCP-3) Standard Curve.

Fig.1. Rat Monocyte chemotactic protein 3 (MCP-3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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