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Rat Mitochondrial-processing peptidase subunit beta (PMPCB) ELISA Kit

Rat Mitochondrial-processing peptidase subunit beta (PMPCB) ELISA Kit

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Specification

Product name Rat Mitochondrial-processing peptidase subunit beta (PMPCB) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Mitochondrial-processing peptidase subunit beta (PMPCB) ELISA Kit employs a two-site sandwich ELISA to quantitate PMPCB in samples. An antibody specific for PMPCB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPMPCB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PMPCB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMPCB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PMPCB; Beta-MPP; MPP11; MPPB; MPPP52; mitochondrial processing peptidase beta subunit

Product Properties

Kit components • Rat Mitochondrial-processing peptidase subunit beta microplate
• Rat Mitochondrial-processing peptidase subunit beta standard
• Rat Mitochondrial-processing peptidase subunit beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Mitochondrial-processing peptidase subunit beta (PMPCB) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PMPCB. No significant cross-reactivity or interference between Rat PMPCB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PMPCb is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.In in vitro assays, MPPB bound frataxin, which was cleaved by the reconstituted MPP heterodimer. MPP cleavage of frataxin resulted in an intermediate form, comprising amino acids 41 to 210, which was processed further to the mature form. In vitro and in vivo experiments suggested that 2 C-terminal missense mutations found in FRDA patients, I151F and G130V, modulated interaction with MPP-beta, resulting in a slower maturation process at the normal cleavage site.
Gene ID 64198
Alternative PMPCB; Beta-MPP; MPP11; MPPB; MPPP52; mitochondrial processing peptidase beta subunit
Accession Q03346

Image & description

Fig.1. Rat Mitochondrial-processing peptidase subunit beta (PMPCB) Standard Curve.

Fig.1. Rat Mitochondrial-processing peptidase subunit beta (PMPCB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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