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Rat Mitochondrial antiviral-signaling protein (MAVS) ELISA Kit

Rat Mitochondrial antiviral-signaling protein (MAVS) ELISA Kit

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Specification

Product name Rat Mitochondrial antiviral-signaling protein (MAVS) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Mitochondrial antiviral-signaling protein (MAVS) ELISA Kit employs a two-site sandwich ELISA to quantitate MAVS in samples. An antibody specific for MAVS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAVS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAVS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAVS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAVS; CARDIF; DKFZp547C224; DKFZp666M015; FLJ27482; FLJ35386; FLJ38051; FLJ41962; IPS-1; IPS1; KIAA1271; MGC3260; VISA; CARD adapter inducing interferon-beta; CARD adaptor inducing IFN-beta; IFN-B promoter

Product Properties

Kit components • Rat Mitochondrial antiviral-signaling protein microplate
• Rat Mitochondrial antiviral-signaling protein standard
• Rat Mitochondrial antiviral-signaling protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Mitochondrial antiviral-signaling protein (MAVS) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAVS. No significant cross-reactivity or interference between Rat MAVS and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK) and IKKB (IKBKB), which activate NFKB , or TBK1 and IKKE (IKBKE), which activate IRF3. Activated IRF3 and NFKB induce transcription of IFNB (IFNB1). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005).Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.
Gene ID 311430
Alternative MAVS; CARDIF; DKFZp547C224; DKFZp666M015; FLJ27482; FLJ35386; FLJ38051; FLJ41962; IPS-1; IPS1; KIAA1271; MGC3260; VISA; CARD adapter inducing interferon-beta; CARD adaptor inducing IFN-beta; IFN-B promoter
Accession Q66HG9

Image & description

Fig.1. Rat Mitochondrial antiviral-signaling protein (MAVS) Standard Curve.

Fig.1. Rat Mitochondrial antiviral-signaling protein (MAVS) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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