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Rat Microtubule-associated serine/threonine-protein kinase 1 (MAST1) ELISA Kit

Rat Microtubule-associated serine/threonine-protein kinase 1 (MAST1) ELISA Kit

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Specification

Product name Rat Microtubule-associated serine/threonine-protein kinase 1 (MAST1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Microtubule-associated serine/threonine-protein kinase 1 (MAST1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAST1 in samples. An antibody specific for MAST1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAST1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAST1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAST1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAST1; KIAA0973; SAST; SAST170; syntrophin associated serine/threonine kinase

Product Properties

Kit components • Rat Microtubule-associated serine/threonine-protein kinase 1 microplate
• Rat Microtubule-associated serine/threonine-protein kinase 1 standard
• Rat Microtubule-associated serine/threonine-protein kinase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Microtubule-associated serine/threonine-protein kinase 1 (MAST1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAST1. No significant cross-reactivity or interference between Rat MAST1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MAST1 shares significant similarity with mouse Mast205 (MAST2). RT-PCR ELISA detected high expression in adult brain, spinal cord, and testis, in fetal brain, and in all specific adult brain regions examined. Expression was moderate in ovary, kidney, and fetal liver, low in pancreas, and very low or undetectable in all other tissues examined. PCR analysis of rat tissues showed that, like Sast170, Sast124 was expressed exclusively in brain. Immunohistochemical analysis detected strong Sast124 staining in neurons of the subventricular zone and granule cells of the olfactory bulb, islands of Calleja, hippocampal dentate gyrus, and cerebellum. Sast124 localized in nuclei of neurons, in glia-like cell bodies in the corpus callosum, and in fiber bundles in the spinal trigeminal and solitary tracts.
Gene ID 353118
Alternative MAST1; KIAA0973; SAST; SAST170; syntrophin associated serine/threonine kinase
Accession Q810W7

Image & description

Fig.1. Rat Microtubule-associated serine/threonine-protein kinase 1 (MAST1) Standard Curve.

Fig.1. Rat Microtubule-associated serine/threonine-protein kinase 1 (MAST1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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