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Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA Kit

Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA Kit

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Specification

Product name Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1LC3B in samples. An antibody specific for MAP1LC3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1LC3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1LC3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1LC3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAP1LC3B; LC3B; MAP1A/1BLC3; microtubule-associated proteins 1A/1B light chain 3

Product Properties

Kit components • Rat Microtubule-associated proteins 1A/1B light chain 3B microplate
• Rat Microtubule-associated proteins 1A/1B light chain 3B standard
• Rat Microtubule-associated proteins 1A/1B light chain 3B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAP1LC3B. No significant cross-reactivity or interference between Rat MAP1LC3B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MAP1LC3b is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.The deduced 125-amino acid protein shares 94% identity with rat Map1lc3. Northern blot analysis detected transcripts of 2.5 and 1.0 kb abundantly expressed in heart, brain, skeletal muscle, and testis, with weaker expression in all other tissues examined. MAP1LC3B was posttranslationally modified. Unlike MAP1LC3A and MAP1LC3C, however, MAP1LC3B did not undergo C-terminal cleavage and did not require the conserved gly120 for posttranslational modification. The essential site for modification of MAP1LC3B was lys122.
Gene ID 64862
Alternative MAP1LC3B; LC3B; MAP1A/1BLC3; microtubule-associated proteins 1A/1B light chain 3
Accession Q62625

Image & description

Fig.1. Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) Standard Curve.

Fig.1. Rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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