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Rat Microtubule-associated protein RP/EB family member 3 (MAPRE3) ELISA Kit

Rat Microtubule-associated protein RP/EB family member 3 (MAPRE3) ELISA Kit

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Specification

Product name Rat Microtubule-associated protein RP/EB family member 3 (MAPRE3) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Microtubule-associated protein RP/EB family member 3 (MAPRE3) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPRE3 in samples. An antibody specific for MAPRE3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPRE3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPRE3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPRE3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAPRE3; EB3; EBF3; EBF3-S; RP3; APC binding protein

Product Properties

Kit components • Rat Microtubule-associated protein RP/EB family member 3 microplate
• Rat Microtubule-associated protein RP/EB family member 3 standard
• Rat Microtubule-associated protein RP/EB family member 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Microtubule-associated protein RP/EB family member 3 (MAPRE3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAPRE3. No significant cross-reactivity or interference between Rat MAPRE3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MAPRE3 is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene. The predicted 282-amino acid protein is 54% identical to MAPRE1. Northern blot analysis revealed expression of a 2.2-kb transcript predominantly in brain and muscle. GST pull-down analysis determined that a homologous region in the C termini of APC and APCL binds to MAPRE3. Immunofluorescence and confocal microscopy demonstrated that MAPRE3 is localized in the microtubule network and colocalizes with APCL in the perinucleus and microtubule network.
Gene ID 298848
Alternative MAPRE3; EB3; EBF3; EBF3-S; RP3; APC binding protein
Accession Q5XIT1

Image & description

Fig.1. Rat Microtubule-associated protein RP/EB family member 3 (MAPRE3) Standard Curve.

Fig.1. Rat Microtubule-associated protein RP/EB family member 3 (MAPRE3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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