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Rat Microtubule-associated protein 4 (MAP4) ELISA Kit

Rat Microtubule-associated protein 4 (MAP4) ELISA Kit

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Specification

Product name Rat Microtubule-associated protein 4 (MAP4) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Microtubule-associated protein 4 (MAP4) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP4 in samples. An antibody specific for MAP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAP4; DKFZp779A1753; MGC8617; OTTHUMP00000210723; OTTHUMP00000210727

Product Properties

Kit components • Rat Microtubule-associated protein 4 microplate
• Rat Microtubule-associated protein 4 standard
• Rat Microtubule-associated protein 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Microtubule-associated protein 4 (MAP4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAP4. No significant cross-reactivity or interference between Rat MAP4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Microtubule-associated protein 4is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules.The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed, the full-length nature of three of which are supported.
Gene ID 367171
Alternative MAP4; DKFZp779A1753; MGC8617; OTTHUMP00000210723; OTTHUMP00000210727
Accession Q5M7W5

Image & description

Fig.1. Rat Microtubule-associated protein 4 (MAP4) Standard Curve.

Fig.1. Rat Microtubule-associated protein 4 (MAP4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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