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Rat Methionine aminopeptidase 2 (METAP2) ELISA Kit

Rat Methionine aminopeptidase 2 (METAP2) ELISA Kit

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Specification

Product name Rat Methionine aminopeptidase 2 (METAP2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Methionine aminopeptidase 2 (METAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate METAP2 in samples. An antibody specific for METAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMETAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for METAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of METAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative METAP2; MAP2; MNPEP; p67; p67eIF2; eIF-2-associated p67 homolog; initiation factor 2-associated 67 kDa glycoprotein; peptidase M 2

Product Properties

Kit components • Rat Methionine aminopeptidase 2 microplate
• Rat Methionine aminopeptidase 2 standard
• Rat Methionine aminopeptidase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Methionine aminopeptidase 2 (METAP2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat METAP2. No significant cross-reactivity or interference between Rat METAP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by METAP2 is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of METAP2 is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of METAP2 have also been shown to be effective for the treatment of obesity. A pseudogene of METAP2 is located on chromosome 2. Several transcript variants encoding different isoforms have been found for METAP2.
Gene ID 64370
Alternative METAP2; MAP2; MNPEP; p67; p67eIF2; eIF-2-associated p67 homolog; initiation factor 2-associated 67 kDa glycoprotein; peptidase M 2
Accession P38062

Image & description

Fig.1. Rat Methionine aminopeptidase 2 (METAP2) Standard Curve.

Fig.1. Rat Methionine aminopeptidase 2 (METAP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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