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Rat Metal Transporter Protein 1 (MTP1) ELISA Kit

Rat Metal Transporter Protein 1 (MTP1) ELISA Kit

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Specification

Product name Rat Metal Transporter Protein 1 (MTP1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Metal Transporter Protein 1 (MTP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC40A1 in samples. An antibody specific for SLC40A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC40A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC40A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC40A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC40A1; FPN1; HFE4; IREG1; MST079; MSTP079; MTP1; SLC11A3; ferroportin 1; iron regulated gene 1; putative ferroportin 1 variantIB; solute carrier family 11 (proton-coupled divalent metal ion transporters);

Product Properties

Kit components • Rat Metal Transporter Protein 1 microplate
• Rat Metal Transporter Protein 1 standard
• Rat Metal Transporter Protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Metal Transporter Protein 1 (MTP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLC40A1. No significant cross-reactivity or interference between Rat SLC40A1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Ferroportin is a transmembrane protein that transports iron from the inside of a cell to the outside of it. Recent research suggests that ferroportin is inhibited by hepcidin, which therefore is the "master regulator" of human iron metabolism. Hepcidin binds to ferroportin, and results in the internalisation of ferroportin within the cell, followed by degradation by the proteasome. This results in retention of iron within the cell, and a reduction in iron levels within the plasma. This is part of the mechanism that causes anaemia of chronic disease; hepcidin is released from the liver in response to inflammatory cytokines, namely interleukin-6, which results in an increased hepcidin concentration and a consequent decrease in plasma iron levels.
Gene ID 170840
Alternative SLC40A1; FPN1; HFE4; IREG1; MST079; MSTP079; MTP1; SLC11A3; ferroportin 1; iron regulated gene 1; putative ferroportin 1 variantIB; solute carrier family 11 (proton-coupled divalent metal ion transporters);
Accession Q923U9

Image & description

Fig.1. Rat Metal Transporter Protein 1 (MTP1) Standard Curve.

Fig.1. Rat Metal Transporter Protein 1 (MTP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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