Login Register
English
0

Cart

$ 0

Rat Meprin A subunit beta (MEP1B) ELISA Kit

Rat Meprin A subunit beta (MEP1B) ELISA Kit

Views(89) Publications(0) Catalog no(KTE100635)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Rat Meprin A subunit beta (MEP1B) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Meprin A subunit beta (MEP1B) ELISA Kit employs a two-site sandwich ELISA to quantitate MEP1B in samples. An antibody specific for MEP1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMEP1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEP1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEP1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MEP1B; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit; PABA peptide hydrolase; PPH beta; endopeptidase-2; meprin A beta

Product Properties

Kit components • Rat Meprin A subunit beta microplate
• Rat Meprin A subunit beta standard
• Rat Meprin A subunit beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Meprin A subunit beta (MEP1B) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MEP1B. No significant cross-reactivity or interference between Rat MEP1B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. MEP1B encodes the beta subunit. Targeted disruption of MEP1B in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine.
Gene ID 25727
Alternative MEP1B; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit; PABA peptide hydrolase; PPH beta; endopeptidase-2; meprin A beta
Accession P28826

Image & description

Fig.1. Rat Meprin A subunit beta (MEP1B) Standard Curve.

Fig.1. Rat Meprin A subunit beta (MEP1B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Rat Meprin A subunit beta (MEP1B) ELISA Kit”