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Rat Mediator of DNA damage checkpoint protein 1 (MDC1) ELISA Kit

Rat Mediator of DNA damage checkpoint protein 1 (MDC1) ELISA Kit

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Specification

Product name Rat Mediator of DNA damage checkpoint protein 1 (MDC1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Mediator of DNA damage checkpoint protein 1 (MDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MDC1 in samples. An antibody specific for MDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MDC1; DAAP-285E11.7; DKFZp781A0122; KIAA0170; MGC166888; NFBD1; homologue to Drosophila photoreceptor protein calphotin; mediator of DNA damage checkpoint 1; nuclear factor with BRCT domains 1

Product Properties

Kit components • Rat Mediator of DNA damage checkpoint protein 1 microplate
• Rat Mediator of DNA damage checkpoint protein 1 standard
• Rat Mediator of DNA damage checkpoint protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Mediator of DNA damage checkpoint protein 1 (MDC1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MDC1. No significant cross-reactivity or interference between Rat MDC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by MDC1 contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
Gene ID 309595
Alternative MDC1; DAAP-285E11.7; DKFZp781A0122; KIAA0170; MGC166888; NFBD1; homologue to Drosophila photoreceptor protein calphotin; mediator of DNA damage checkpoint 1; nuclear factor with BRCT domains 1
Accession Q5U2M8

Image & description

Fig.1. Rat Mediator of DNA damage checkpoint protein 1 (MDC1) Standard Curve.

Fig.1. Rat Mediator of DNA damage checkpoint protein 1 (MDC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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