Login Register
English
0

Cart

$ 0

Rat Matrix metalloproteinase-24 (MMP24) ELISA Kit

Rat Matrix metalloproteinase-24 (MMP24) ELISA Kit

Views(24) Publications(0) Catalog no(KTE100603)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Rat Matrix metalloproteinase-24 (MMP24) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Matrix metalloproteinase-24 (MMP24) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP24 in samples. An antibody specific for MMP24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP24 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP24 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP24 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MMP24; RP4-614O4.10; MMP25; MT-MMP5; MT5-MMP; matrix metalloproteinase 24; matrix metalloproteinase 24 (membrane-inserted); membrane-type 5 matrix metalloproteinase

Product Properties

Kit components • Rat Matrix metalloproteinase-24 microplate
• Rat Matrix metalloproteinase-24 standard
• Rat Matrix metalloproteinase-24 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Matrix metalloproteinase-24 (MMP24) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MMP24. No significant cross-reactivity or interference between Rat MMP24 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MMP24 encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. Unlike most MMPs, which are secreted, this protease is a member of the membrane-type MMP (MT-MMP) subfamily, contains a transmembrane domain and is expressed at the cell surface. Substrates of this protease include the proteins cadherin 2 and matrix metallopeptidase 2 (also known as 72 kDa type IV collagenase).
Gene ID 83513
Alternative MMP24; RP4-614O4.10; MMP25; MT-MMP5; MT5-MMP; matrix metalloproteinase 24; matrix metalloproteinase 24 (membrane-inserted); membrane-type 5 matrix metalloproteinase
Accession Q99PW6

Image & description

Fig.1. Rat Matrix metalloproteinase-24 (MMP24) Standard Curve.

Fig.1. Rat Matrix metalloproteinase-24 (MMP24) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Rat Matrix metalloproteinase-24 (MMP24) ELISA Kit”