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Rat Matrix metalloproteinase 10 (MMP-10) ELISA Kit

Rat Matrix metalloproteinase 10 (MMP-10) ELISA Kit

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Specification

Product name Rat Matrix metalloproteinase 10 (MMP-10) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Matrix metalloproteinase 10 (MMP-10) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP10 in samples. An antibody specific for MMP10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MMP10; SL-2; STMY2; matrix metalloprotease 10; matrix metalloproteinase 10; matrix metalloproteinase 10 (stromelysin 2); stromelysin 2; transin 2

Product Properties

Kit components • Rat Matrix metalloproteinase 10 microplate
• Rat Matrix metalloproteinase 10 standard
• Rat Matrix metalloproteinase 10 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Matrix metalloproteinase 10 (MMP-10) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MMP10. No significant cross-reactivity or interference between Rat MMP10 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MMP10 encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down fibronectin, laminin, elastin, proteoglycan core protein, gelatins, and several types of collagen. MMP10 is part of a cluster of MMP genes on chromosome 11.
Gene ID 117061
Alternative MMP10; SL-2; STMY2; matrix metalloprotease 10; matrix metalloproteinase 10; matrix metalloproteinase 10 (stromelysin 2); stromelysin 2; transin 2
Accession P07152

Image & description

Fig.1. Rat Matrix metalloproteinase 10 (MMP-10) Standard Curve.

Fig.1. Rat Matrix metalloproteinase 10 (MMP-10) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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