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Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) ELISA Kit

Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) ELISA Kit

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Specification

Product name Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAN1A1 in samples. An antibody specific for MAN1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAN1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAN1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAN1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAN1A1; HUMM3; HUMM9; MAN9; Man9-mannosidase; alpha-1;2-mannosidase IA; mannosyl-oligosaccharide 1;2-alpha-mannosidase IA N/A

Product Properties

Kit components • Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA microplate
• Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA standard
• Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAN1A1. No significant cross-reactivity or interference between Rat MAN1A1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MAN1A1 encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes the removal of 3 distinct mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. Using an oligonucleotide probe derived from a pig liver Man(9)-mannosidase-specific cDNA template, Bause et al. (1993) isolated Man(9)-mannosidase from a human kidney cDNA library. The full-length cDNA predicted a 625-amino acid protein with a calculated molecular mass of 71 kD. Man(9)-mannosidase is a type II transmembrane protein with a short cytoplasmic polypeptide tail, a single transmembrane domain acting as a noncleavable signal sequence, a large luminal catalytic domain, and 3 potential N-glycosylation sites.
Alternative MAN1A1; HUMM3; HUMM9; MAN9; Man9-mannosidase; alpha-1;2-mannosidase IA; mannosyl-oligosaccharide 1;2-alpha-mannosidase IA N/A

Image & description

Fig.1. Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) Standard Curve.

Fig.1. Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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