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Rat MAM domain-containing glycosylphosphatidylinositol anchor protein 1 (MDGA1) ELISA Kit

Rat MAM domain-containing glycosylphosphatidylinositol anchor protein 1 (MDGA1) ELISA Kit

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Specification

Product name Rat MAM domain-containing glycosylphosphatidylinositol anchor protein 1 (MDGA1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat MAM domain-containing glycosylphosphatidylinositol anchor protein 1 (MDGA1) ELISA Kit employs a two-site sandwich ELISA to quantitate MDGA1 in samples. An antibody specific for MDGA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDGA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDGA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDGA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MDGA1; DKFZp686K0262; DKFZp686L0262; FLJ45018; GPIM; MAMDC3; glycosyl-phosphatidyl-inositol-MAM

Product Properties

Kit components • Rat MAM domain-containing glycosylphosphatidylinositol anchor protein 1 microplate
• Rat MAM domain-containing glycosylphosphatidylinositol anchor protein 1 standard
• Rat MAM domain-containing glycosylphosphatidylinositol anchor protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat MAM domain-containing glycosylphosphatidylinositol anchor protein 1 (MDGA1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MDGA1. No significant cross-reactivity or interference between Rat MDGA1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MDGA1 encodes a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that is expressed predominantly in the developing nervous system. In addition to possessing several cell adhesion molecule-like domains, the mature protein has six Ig-like domains, a single fibronectin type III domain, a MAM domain and a C-terminal GPI-anchoring site. Studies in other mammals suggest this protein plays a role in cell adhesion, migration, and axon guidance and, in the developing brain, neuronal migration. In humans, MDGA1 is associated with bipolar disorder and schizophrenia.
Gene ID 309659
Alternative MDGA1; DKFZp686K0262; DKFZp686L0262; FLJ45018; GPIM; MAMDC3; glycosyl-phosphatidyl-inositol-MAM
Accession P85171

Image & description

Fig.1. Rat MAM domain-containing glycosylphosphatidylinositol anchor protein 1 (MDGA1) Standard Curve.

Fig.1. Rat MAM domain-containing glycosylphosphatidylinositol anchor protein 1 (MDGA1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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