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Rat MAGUK p55 subfamily member 3 (MPP3) ELISA Kit

Rat MAGUK p55 subfamily member 3 (MPP3) ELISA Kit

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Specification

Product name Rat MAGUK p55 subfamily member 3 (MPP3) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat MAGUK p55 subfamily member 3 (MPP3) ELISA Kit employs a two-site sandwich ELISA to quantitate MPP3 in samples. An antibody specific for MPP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MPP3; DLG3; MAGUK p55 subfamily member 3; discs; large homolog 3; membrane protein palmitoylated 3; palmitoylated membrane protein 3

Product Properties

Kit components • Rat MAGUK p55 subfamily member 3 microplate
• Rat MAGUK p55 subfamily member 3 standard
• Rat MAGUK p55 subfamily member 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat MAGUK p55 subfamily member 3 (MPP3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MPP3. No significant cross-reactivity or interference between Rat MPP3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MPP3 product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein.
Gene ID 114202
Alternative MPP3; DLG3; MAGUK p55 subfamily member 3; discs; large homolog 3; membrane protein palmitoylated 3; palmitoylated membrane protein 3
Accession O88954

Image & description

Fig.1. Rat MAGUK p55 subfamily member 3 (MPP3) Standard Curve.

Fig.1. Rat MAGUK p55 subfamily member 3 (MPP3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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