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Rat Macrophage Migration Inhibitory Factor (MIF) ELISA Kit

Rat Macrophage Migration Inhibitory Factor (MIF) ELISA Kit

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Specification

Product name Rat Macrophage Migration Inhibitory Factor (MIF) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Macrophage Migration Inhibitory Factor (MIF) ELISA Kit employs a two-site sandwich ELISA to quantitate MIF in samples. An antibody specific for MIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMIF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MIF; GIF; GLIF; MMIF; macrophage migration inhibitory factor; phenylpyruvate tautomerase

Product Properties

Kit components • Rat Macrophage Migration Inhibitory Factor microplate
• Rat Macrophage Migration Inhibitory Factor standard
• Rat Macrophage Migration Inhibitory Factor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Macrophage Migration Inhibitory Factor (MIF) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MIF. No significant cross-reactivity or interference between Rat MIF and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MIF encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.
Gene ID 103694877
Alternative MIF; GIF; GLIF; MMIF; macrophage migration inhibitory factor; phenylpyruvate tautomerase
Accession P30904

Image & description

Fig.1. Rat Macrophage Migration Inhibitory Factor (MIF) Standard Curve.

Fig.1. Rat Macrophage Migration Inhibitory Factor (MIF) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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