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Rat Macrophage erythroblast attacher (MAEA) ELISA Kit

Rat Macrophage erythroblast attacher (MAEA) ELISA Kit

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Specification

Product name Rat Macrophage erythroblast attacher (MAEA) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Macrophage erythroblast attacher (MAEA) ELISA Kit employs a two-site sandwich ELISA to quantitate MAEA in samples. An antibody specific for MAEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAEA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAEA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAEA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAEA; EMLP; EMP; HLC-10; PIG5; erythroblast macrophage protein; lung cancer-related protein 10; proliferation-inducing gene 5

Product Properties

Kit components • Rat Macrophage erythroblast attacher microplate
• Rat Macrophage erythroblast attacher standard
• Rat Macrophage erythroblast attacher detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Macrophage erythroblast attacher (MAEA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAEA. No significant cross-reactivity or interference between Rat MAEA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Macrophage Erythroblast Attacher mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.
Gene ID 298982
Alternative MAEA; EMLP; EMP; HLC-10; PIG5; erythroblast macrophage protein; lung cancer-related protein 10; proliferation-inducing gene 5
Accession Q5RKJ1

Image & description

Fig.1. Rat Macrophage erythroblast attacher (MAEA) Standard Curve.

Fig.1. Rat Macrophage erythroblast attacher (MAEA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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