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Rat Lipoprotein-associated phospholipase A2 (Lp-PLA2) ELISA Kit

Rat Lipoprotein-associated phospholipase A2 (Lp-PLA2) ELISA Kit

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Specification

Product name Rat Lipoprotein-associated phospholipase A2 (Lp-PLA2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Lipoprotein-associated phospholipase A2 (Lp-PLA2) ELISA Kit employs a two-site sandwich ELISA to quantitate Lp-PLA2 in samples. An antibody specific for Lp-PLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLp-PLA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Lp-PLA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Lp-PLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative Lp-PLA2; LDL-PLA2; PLA2G7; PAFAH; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; PAF 2-acylhydrolase; lipoprotein-associated phospholipase A2; phospholipase A2;

Product Properties

Kit components • Rat Lipoprotein-associated phospholipase A2 microplate
• Rat Lipoprotein-associated phospholipase A2 standard
• Rat Lipoprotein-associated phospholipase A2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Lipoprotein-associated phospholipase A2 (Lp-PLA2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat Lp-PLA2. No significant cross-reactivity or interference between Rat Lp-PLA2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Lipoprotein-associated phospholipase A2 (Lp-PLA2, also known as platelet activating factor acetyl-hydrolase), is a relatively recent marker that has emerged as a major, independent predictor of CHD. Lp-PLA2 is an active phospholipase that circulates in plasma bound to LDL, and it appears to act almost exclusively on oxidized LDL to generate lysophosphatidyl-choline, which is proinflammatory and is considered to be atherogenic. Thus, Lp-PLA2 represents an interesting link between lipoproteinoxidation and vascular inflammation, which likely explain its strong, independent association with CHD risk. Data from the recent ARIC study have indicated that the predictive power of Lp-PLA2 is attenuated after adjustment for multiple established risk factors.
Alternative Lp-PLA2; LDL-PLA2; PLA2G7; PAFAH; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; PAF 2-acylhydrolase; lipoprotein-associated phospholipase A2; phospholipase A2;

Image & description

Fig.1. Rat Lipoprotein-associated phospholipase A2 (Lp-PLA2) Standard Curve.

Fig.1. Rat Lipoprotein-associated phospholipase A2 (Lp-PLA2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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