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Rat Leukemia inhibitory factor (LIF) ELISA Kit

Rat Leukemia inhibitory factor (LIF) ELISA Kit

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Specification

Product name Rat Leukemia inhibitory factor (LIF) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Leukemia inhibitory factor (LIF) ELISA Kit employs a two-site sandwich ELISA to quantitate LIF in samples. An antibody specific for LIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLIF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LIF; CDF; DIA; HILDA; D factor; cholinergic differentiation factor; differentiation inhibitory activity; differentiation stimulating factor

Product Properties

Kit components • Rat Leukemia inhibitory factor microplate
• Rat Leukemia inhibitory factor standard
• Rat Leukemia inhibitory factor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Leukemia inhibitory factor (LIF) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LIF. No significant cross-reactivity or interference between Rat LIF and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Leukemia inhibitory factor, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukaemic cells. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF is normally expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. As embryonic stem cells are derived from the inner cell mass at the blastocyst stage, removing them from the inner cell mass also removes their source of LIF.
Alternative LIF; CDF; DIA; HILDA; D factor; cholinergic differentiation factor; differentiation inhibitory activity; differentiation stimulating factor
Accession P17777

Image & description

Fig.1. Rat Leukemia inhibitory factor (LIF) Standard Curve.

Fig.1. Rat Leukemia inhibitory factor (LIF) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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