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Rat Leucine aminopepridase (LAP) ELISA Kit

Rat Leucine aminopepridase (LAP) ELISA Kit

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Specification

Product name Rat Leucine aminopepridase (LAP) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Leucine aminopepridase (LAP) ELISA Kit employs a two-site sandwich ELISA to quantitate LAP3 in samples. An antibody specific for LAP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LAP3; LAP; LAPEP; PEPS; peptidase S

Product Properties

Kit components • Rat Leucine aminopepridase microplate
• Rat Leucine aminopepridase standard
• Rat Leucine aminopepridase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Leucine aminopepridase (LAP) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LAP3. No significant cross-reactivity or interference between Rat LAP3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Leucine aminopeptidase (LAP) is an exopeptidase hydrolyzing the peptide bond adjacent to a free amino group. LAP is extensively used in the sequence analysis of proteins and peptides. .The enzyme liberates amino acids from the N-terminal end of a number of proteins and polypeptides. It is called leucine aminopeptidase because it reacts most rapidly with leucine compounds. Many aliphatic amides are also hydrolyzed as well as thiolesters.LAP consists of four subunits each having one atom of zinc. PEPS levels did not differ from controls in any. This experience permitted exclusion mapping of PEPS to 4p11-4q13; combined with previously reported data, the assignment becomes 4p11-4q12.
Gene ID 289668
Alternative LAP3; LAP; LAPEP; PEPS; peptidase S
Accession Q68FS4

Image & description

Fig.1. Rat Leucine aminopepridase (LAP) Standard Curve.

Fig.1. Rat Leucine aminopepridase (LAP) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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