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Rat Lecithin Cholesterol Acyltransferase (LCAT) ELISA Kit

Rat Lecithin Cholesterol Acyltransferase (LCAT) ELISA Kit

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Specification

Product name Rat Lecithin Cholesterol Acyltransferase (LCAT) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Lecithin Cholesterol Acyltransferase (LCAT) ELISA Kit employs a two-site sandwich ELISA to quantitate LCAT in samples. An antibody specific for LCAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLCAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LCAT; Phosphatidylcholine-sterol acyltransferase

Product Properties

Kit components • Rat Lecithin Cholesterol Acyltransferase microplate
• Rat Lecithin Cholesterol Acyltransferase standard
• Rat Lecithin Cholesterol Acyltransferase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Lecithin Cholesterol Acyltransferase (LCAT) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LCAT. No significant cross-reactivity or interference between Rat LCAT and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Lecithin-cholesterol acyltransferase is an enzyme that converts free cholesterol into cholesteryl ester (a more hydrophobic form of cholesterol), which is then sequestered into the core of a lipoprotein particle, eventually making the newly synthesized HDL spherical and forcing the reaction to become unidirectional since the particles are removed from the surface. The enzyme is bound to high-density lipoproteins (HDLs) and low-density lipoproteins in the blood plasma. An unusual feature of the message is that the poly(A) signal appears to overlap the COOH-terminal glutamic acid and stop codons. The protein has several extended sequences of hydrophobic amino acids, one of which is similar to sequences in pancreatic lipase and lingual lipase.
Gene ID 100008978
Alternative LCAT; Phosphatidylcholine-sterol acyltransferase
Accession P53761

Image & description

Fig.1. Rat Lecithin Cholesterol Acyltransferase (LCAT) Standard Curve.

Fig.1. Rat Lecithin Cholesterol Acyltransferase (LCAT) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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