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Rat Laminin subunit gamma-2 (LAMC2) ELISA Kit

Rat Laminin subunit gamma-2 (LAMC2) ELISA Kit

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Specification

Product name Rat Laminin subunit gamma-2 (LAMC2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Laminin subunit gamma-2 (LAMC2) ELISA Kit employs a two-site sandwich ELISA to quantitate LAMC2 in samples. An antibody specific for LAMC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LAMC2; B2T; BM600; CSF; EBR2; EBR2A; LAMB2T; LAMNB2; MGC138491; MGC141938; BM600-100kDa; cell-scattering factor (140kDa); epiligrin; kalinin (105kD); kalinin-105kDa; ladsin (140kDa); laminin; gamma 2 (nicein (10

Product Properties

Kit components • Rat Laminin subunit gamma-2 microplate
• Rat Laminin subunit gamma-2 standard
• Rat Laminin subunit gamma-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Laminin subunit gamma-2 (LAMC2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LAMC2. No significant cross-reactivity or interference between Rat LAMC2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background LAMC2 is the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5, which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized.
Alternative LAMC2; B2T; BM600; CSF; EBR2; EBR2A; LAMB2T; LAMNB2; MGC138491; MGC141938; BM600-100kDa; cell-scattering factor (140kDa); epiligrin; kalinin (105kD); kalinin-105kDa; ladsin (140kDa); laminin; gamma 2 (nicein (10

Image & description

Fig.1. Rat Laminin subunit gamma-2 (LAMC2) Standard Curve.

Fig.1. Rat Laminin subunit gamma-2 (LAMC2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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