|Product name||Rat Laminin alpha 1 (LAMA1) ELISA Kit|
|Applications notes||This Rat Laminin alpha 1 (LAMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate LAMA1 in samples. An antibody specific for LAMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Laminin alpha 1 microplate
• Rat Laminin alpha 1 standard
• Rat Laminin alpha 1 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Laminin alpha 1 (LAMA1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LAMA1. No significant cross-reactivity or interference between Rat LAMA1 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Laminin is a basement membrane protein composed of 3 nonidentical chains, A (400 kD), B1 (230 kD), and B2 (220 kD), which are arranged in a cross-shaped structure. Mattei et al. (1989) used in situ hybridization to localize the human laminin A locus to chromosome 18p11.31.By study of mouse-Chinese hamster somatic cell hybrids, Kaye et al. assigned the gene for laminin A to mouse chromosome 17 and confirmed the assignment of the gene for laminin B2 to mouse chromosome 1. Haaparanta et al. reported the nucleotide sequence of LAMA cDNA. They found an open reading frame encoding 3,075 amino acids. Nissinen et al. reported that the complete human laminin A chain contains a 17-amino acid signal peptide and a 3,058-residue A chain proper. The chain has a distinct domain structure with numerous internal cysteine-rich repeats.|
Fig.1. Rat Laminin alpha 1 (LAMA1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.