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Rat Krueppel-like factor 2 (KLF2) ELISA Kit

Rat Krueppel-like factor 2 (KLF2) ELISA Kit

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Specification

Product name Rat Krueppel-like factor 2 (KLF2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Krueppel-like factor 2 (KLF2) ELISA Kit employs a two-site sandwich ELISA to quantitate KLF2 in samples. An antibody specific for KLF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative KLF2; LKLF; Kruppel-like factor; Kruppel-like factor LKLF; lung Kruppel-like zinc finger transcription factor

Product Properties

Kit components • Rat Krueppel-like factor 2 microplate
• Rat Krueppel-like factor 2 standard
• Rat Krueppel-like factor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Krueppel-like factor 2 (KLF2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KLF2. No significant cross-reactivity or interference between Rat KLF2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Mature single-positive (CD4+ or CD8+) T lymphocytes enter a 'resting' state in which they are proliferatively quiescent and relatively resistant to apoptosis. Anderson et al. (1995) identified and characterized the gene for a novel zinc finger transcription factor that they symbolized LKLF for 'lung Kruppel-like factor.' Using 5-prime and 3-prime RACE on a melted GC-rich DNA fragment similar to mouse Lklf, Kozyrev et al. (1999) isolated a cDNA encoding KLF2, which they termed LKLF. Sequence analysis predicted that the 355-amino acid LKLF protein, which is 87% identical to the mouse protein, has N-terminal proline-rich repeats, a putative activation domain, a potential nuclear localization signal, and a C-terminal zinc finger domain.
Gene ID 306330
Alternative KLF2; LKLF; Kruppel-like factor; Kruppel-like factor LKLF; lung Kruppel-like zinc finger transcription factor
Accession Q9ET58

Image & description

Fig.1. Rat Krueppel-like factor 2 (KLF2) Standard Curve.

Fig.1. Rat Krueppel-like factor 2 (KLF2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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